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Why All Men Should be Tested with the Sensitive Estradiol Test
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CoastWatcher
Moderator
Knut Erik Knutsen
Member
Can you say if this is an Sensitive E2 test:
ABSTRACT In this simple, reliable, fast solid-phase chemiluminescence immunoassay for directly measuring (i.e., without prior extraction) estradiol-17 beta in serum, a monoclonal antibody is used that binds estradiol with high affinity (Ka = 10(10) L/mol), and does not bind other steroids tested, the highest cross reactivity observed being 0.1% for estradiol-17 alpha. In this system the monoclonal antibody is bound to the wells of microtiter plates via a second antibody directed against the monoclonal antibody. Fifty microliters of serum and estradiol-displacing agents are added, followed by 100 pg of estradiol-isoluminol conjugate, and the label is measured by luminometry after the binding reaction. The sensitivity of the assay is 180 pmol per liter of serum, and the effective working range at less than or equal to 10% CV is 270 to 6700 pmol/L. Analytical recovery of added estradiol averaged 99.7% (SD 6.5%). Within- and between-assay CVs ranged between 5 and 12.7%. Thirty-five unknown serum samples can be assayed within 4 h. Results correlated well with those obtained with a direct RIA: r = 0.94 (n = 149). This assay opens new perspectives for chemiluminescence immunoassays.
Direct chemiluminescence immunoassay for estradiol in serum (PDF Download Available). Available from: http://www.researchgate.net/publica...minescence_immunoassay_for_estradiol_in_serum [accessed Aug 7, 2015].
ABSTRACT In this simple, reliable, fast solid-phase chemiluminescence immunoassay for directly measuring (i.e., without prior extraction) estradiol-17 beta in serum, a monoclonal antibody is used that binds estradiol with high affinity (Ka = 10(10) L/mol), and does not bind other steroids tested, the highest cross reactivity observed being 0.1% for estradiol-17 alpha. In this system the monoclonal antibody is bound to the wells of microtiter plates via a second antibody directed against the monoclonal antibody. Fifty microliters of serum and estradiol-displacing agents are added, followed by 100 pg of estradiol-isoluminol conjugate, and the label is measured by luminometry after the binding reaction. The sensitivity of the assay is 180 pmol per liter of serum, and the effective working range at less than or equal to 10% CV is 270 to 6700 pmol/L. Analytical recovery of added estradiol averaged 99.7% (SD 6.5%). Within- and between-assay CVs ranged between 5 and 12.7%. Thirty-five unknown serum samples can be assayed within 4 h. Results correlated well with those obtained with a direct RIA: r = 0.94 (n = 149). This assay opens new perspectives for chemiluminescence immunoassays.
Direct chemiluminescence immunoassay for estradiol in serum (PDF Download Available). Available from: http://www.researchgate.net/publica...minescence_immunoassay_for_estradiol_in_serum [accessed Aug 7, 2015].
sportsfan8724
Member
Did you get your testosterone levels also? Are you on trt?
Yes, I've had testosterone checked many times and it is low. I'm not on TRT. Just wondering what an "optimal" estradiol level is for men.
Thanks!
CoastWatcher
Moderator
An optimal level of testosterone is an individual value. I am quite comfortable if, on the sensitive test, my range is between 29 and 37. Others do well with slightly higher or slightly lower numbers. The sensitive result is the beginning if the discussion that a good doctor and his patient have. Other considerations are total and free testosterone levels, the testosterone:estrogen ratio, symptoms, and - most importantly - how the patient feels. These factors determine the answer.
Well, I understand that it varies for each individual. What I am wanting to know, is if a level of 11 (on a scale 8-35) would or could cause additional symptoms?
Obviously, since I have many symptoms, I'm assuming most of them are from low test, (lowest level 154) but I'm wondering if I am experiencing symptoms from low estradiol as well.
Obviously, since I have many symptoms, I'm assuming most of them are from low test, (lowest level 154) but I'm wondering if I am experiencing symptoms from low estradiol as well.
CoastWatcher
Moderator
Well, that is an objectively low estradiol value. With low testosterone it isn't necessarily a surprise that you have low E. It could well be a factor in how you feel.
Vince
Super Moderator
You should post full labs with rangers, under the Blood Test Discussion thread. https://www.excelmale.com/forumdisplay.php?58-Blood-Test-Discussion
Sorry to bump this thread but which LabCorp estradiol test should we be using? 140244 or 500108? They are both LC/MS-MS.
https://www.labcorp.com/test-menu/search?query=Estradiol
https://www.labcorp.com/test-menu/search?query=Estradiol
CoastWatcher
Moderator
LabCorp 140244
CoastWatcher
Moderator
What other changes, if any, in your protocol, might have been made?
None really. Maybe i never needed the inhibitor. My dr goes by the standard test.
Originally Posted by CoastWatcher 
What other changes, if any, in your protocol, might have been made?None really. Maybe i never needed the inhibitor. My dr goes by the standard test. "
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Are you saying that you ran the SENSTITIVE TEST and results was 17 (8.0-35.0 range) and then ran it again after a few days and it was 5 (8.0-35.0)?
If your Endo has been prescribing an AI based on the STANDARD test you could easily overcorrect and go waaaaay to low.
https://www.labcorp.com/test-menu/24871/estradiol-sensitive-lc-ms
Adult Men. The use of a sensitive, LC/MS assay for serum E2 measurement in males is preferred over direct immunoassays because of its greater sensitivity and lesser interference by other steroids.[SUP]28[/SUP] In males, estradiol is present at low concentrations in blood, but it is extraordinarily high in semen.[SUP]4[/SUP] Estradiol plays an important role in epididymal function and sperm maturation and is essential for normal spermatogenesis and sperm motility.[SUP]4[/SUP]
None really. Maybe i never needed the inhibitor. My dr goes by the standard test. "
***********************************************************************************
Are you saying that you ran the SENSTITIVE TEST and results was 17 (8.0-35.0 range) and then ran it again after a few days and it was 5 (8.0-35.0)?
If your Endo has been prescribing an AI based on the STANDARD test you could easily overcorrect and go waaaaay to low.
https://www.labcorp.com/test-menu/24871/estradiol-sensitive-lc-ms
Adult Men. The use of a sensitive, LC/MS assay for serum E2 measurement in males is preferred over direct immunoassays because of its greater sensitivity and lesser interference by other steroids.[SUP]28[/SUP] In males, estradiol is present at low concentrations in blood, but it is extraordinarily high in semen.[SUP]4[/SUP] Estradiol plays an important role in epididymal function and sperm maturation and is essential for normal spermatogenesis and sperm motility.[SUP]4[/SUP]
That is correct. I will not keep taking the e2 until I get retested with the sensitive again. Like I said earlier maybe I never needed the e2 blocker. I don't care what number the standard is so long as the sensitive is normal.
I suggest that you get a copy of Lee Myer's book ASAP...... And do a little reading to get a better understanding of the whole T and E2 issue (plus other stuff).
https://www.excelmale.com/forum/sho...VS-Testosterone-Therapy-by-Lee-Myer-Recommend
https://www.excelmale.com/forum/sho...VS-Testosterone-Therapy-by-Lee-Myer-Recommend
Thanks. Idk if I've benefited from trt. I may just quit.
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