CDC STANDARDIZED TOTAL T and ESTRADIOL ASSAYS and soon to be FREE TESTOSTERONE!

[madman]

CDC Hormone Standardization Program (CDC HoSt) Certified Estradiol Assays (UPDATED 4/2024)

 

[madman]

Comparability of Free Testosterone Measurements- Results of the Interlaboratory Study

https://academic.oup.com/clinchem/article/70/Supplement_1/hvae106.074/7760719 Assessment of free testosterone (FT) has been recommended as a biomarker for diagnosis and management of hypogonadism and female hyperandrogenism. Reliable laboratory measurements are essential for correctly...

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[madman]

* The data from the present analyses suggest that the interaction of the three sex hormones with their cognate binding proteins is highly complex and dynamic and influenced by their relative circulating concentrations. Therefore, models of testosterones binding to SHBG, based on the assumption of fixed apparent binding affinity of sex hormones with SHBG, that do not consider the influence of estradiol and dihydrotestosterone on the free testosterone fraction are unlikely to provide accurate estimates of free testosterone fraction.

* Because of these complex interactions between various sex hormones as well as other ligands with sex hormone binding globulin, direct measurements of free testosterone using a reliable assay, such as the equilibrium dialysis method, may be a superior marker of testosterone’s treatment effect.

Modulation of circulating free testosterone fraction by T, DHT and estradiol during TRT

The thread discusses the modulation of circulating free testosterone fraction during Testosterone Replacement Therapy (TRT) and its relationship with other hormones. Here are the main points from the discussion: Free Testosterone Fraction: The thread emphasizes the importance of the free...

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* Collectively, these data highlight the non-linear, concentration-dependent modulation of testosterone repartitioning into bound and free fractions by each of the three sex hormones.

*Our finding that the estradiol, DHT, and testosterone interact to alter free testosterone fraction non-linearly suggests that in men with hypogonadism who are receiving TRT, free testosterone levels should be measured using a reliable method to guide the dose titration. The models that do not consider changes in estradiol and DHT concentrations are susceptible to error in estimating free testosterone concentrations.

*These data suggest that changes in estradiol and dihydrotestosterone concentrations should be considered in evaluating response to testosterone treatment because of their differential influence on free testosterone concentrations in addition to their ability to exert other independent biologic effects. Because of these complex interactions between various sex hormones as well as other ligands with sex hormone binding globulin, direct measurements of free testosterone using a reliable assay, such as the equilibrium dialysis method, may be a superior marker of testosterone’s treatment effect.

 

[madman]

CDC Hormone Standardization Program (CDC HoSt) Certified Total Testosterone Procedures (UPDATED 9/2024)


Siemens Healthcare are the only ones with a CDC HoSt Certified TT Chemiluminescence Immunoassays (Atellica IM TSTII, ADVIA Centaur TSTII, ADVIA Centaur CP TSTII, Dimension Vista TTST)!

Siemens Healthineers is the only T immunoassay manufacturer to be currently certified CDC HoST

Authors C. Abou-Diwan1, N. Parker, S. Pagliaro, S. Lewisch, E. Barczak , V. Shalhoub, E. Oduaran, J. Freeman. Siemens Healthineers, Tarrytown, NY, Siemens Healthcare Diagnostics Inc., Tarrytown, NY, Abstract Background The CDC Hormone Standardization (CDC HoST) Certification Program, is...

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Conclusions

The TSTII assay consistently achieved CDC certification from 2019 to 2023, while the TTST assay was initially certified in 2014. Siemens Healthineers is the only testosterone immunoassay manufacturer to be currently certified, the TSTII assay has achieved certification for four consecutive years.

 

[madman]

CDC Hormone Standardization Program (CDC HoSt) Certified Estradiol Assays (UPDATED 9/2024)


Quest Diagnostics still nowhere to be found!

 

[madman]

* The data from the present analyses suggest that the interaction of the three sex hormones with their cognate binding proteins is highly complex and dynamic and influenced by their relative circulating concentrations. Therefore, models of testosterones binding to SHBG, based on the assumption of fixed apparent binding affinity of sex hormones with SHBG, that do not consider the influence of estradiol and dihydrotestosterone on the free testosterone fraction are unlikely to provide accurate estimates of free testosterone fraction.

* Because of these complex interactions between various sex hormones as well as other ligands with sex hormone binding globulin, direct measurements of free testosterone using a reliable assay, such as the equilibrium dialysis method, may be a superior marker of testosterone’s treatment effect.

Modulation of circulating free testosterone fraction by T, DHT and estradiol during TRT

The thread discusses the modulation of circulating free testosterone fraction during Testosterone Replacement Therapy (TRT) and its relationship with other hormones. Here are the main points from the discussion: Free Testosterone Fraction: The thread emphasizes the importance of the free...

www.excelmale.com

* Collectively, these data highlight the non-linear, concentration-dependent modulation of testosterone repartitioning into bound and free fractions by each of the three sex hormones.

*Our finding that the estradiol, DHT, and testosterone interact to alter free testosterone fraction non-linearly suggests that in men with hypogonadism who are receiving TRT, free testosterone levels should be measured using a reliable method to guide the dose titration. The models that do not consider changes in estradiol and DHT concentrations are susceptible to error in estimating free testosterone concentrations.

*These data suggest that changes in estradiol and dihydrotestosterone concentrations should be considered in evaluating response to testosterone treatment because of their differential influence on free testosterone concentrations in addition to their ability to exert other independent biologic effects. Because of these complex interactions between various sex hormones as well as other ligands with sex hormone binding globulin, direct measurements of free testosterone using a reliable assay, such as the equilibrium dialysis method, may be a superior marker of testosterone’s treatment effect.

* The values derived from the random forest model exhibited high level of concordance with the laboratory-measured values of percent free testosterone ( R² 0.78, p= 2.2 × 10-74), confirming the robustness of the analytical model (Figure 1).