lc-ms/ms

MSACL 2025 Abstract Matthew R Chappell (1), Meghan N Bradley (1), Matthew L Crawford (1), Sven Groesgen (2), Philippe Metz (2), Russell P Grant (1) (1) Center for Esoteric Testing, Labcorp, Burlington NC 27215, (2) Roche Diagnostics GmbH, Mannheim, Germany Abstract INTRODUCTION The...

* Probably the most challenging forms to quantify, due to their relatively low concentrations are the so called free forms. The free forms of all the hormones (shown in Figs. 1–3) are the hormones not bound to proteins, or conjugated to other molecules (see Fig. 2) and are biologically active...

* A standardized ED method is recommended to effectively separate FT from the serum matrix. * With the rapid development of liquid chromatography-tandem mass spectrometry (LC-MS/MS), the ED separation combined with LC-MS/MS has become the “gold standard” for quantifying FT [4]. * Serum FT...

Abstract Objectives A mass spectrometry (LC-MS/MS)--based interlaboratory comparison study was performed for nine steroid analytes with five participating laboratories. The sample set contained 40 pooled samples of human serum generated from preanalyzed leftovers. To obtain a well-balanced...

Serum INSL3, LH, and testosterone levels were studied in different contexts. After Sustanon treatment, testosterone initially increased but then rapidly decreased due to clearance and suppressed secretion. INSL3 complemented testosterone measurements, reflecting immediate Leydig cell function...

ABSTRACT Accurate measurement of testosterone is important for the diagnosis of gonadal disorders in men, women, and children. Testosterone measurement has limited accuracy at low concentrations by most commercially available immunoassays. We aimed to develop an LC-MS/MS assay to address the...

Development and validation of an LC-MS/MS assay for the quantification of allopregnanolone and its progesterone-derived isomers, precursors, and cortisol/cortisone in pregnancy Abstract Neuroactive steroids are potent neuromodulators that play a critical role in both maternal and fetal health...

Is Total T MS panel specific enough to recognize a testosterone molecule from a nandrolone molecule? I know the standard non sensitive Total T panel can’t differentiate test and nandrolone and will add them together, and the Total T LC/MS/MS panel is specific enough to know the difference and...

Abstract Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful tool that is changing the way we analyze steroids in the clinical laboratory. It offers positive compound identification to enable the unequivocal identification of a compound free from interference. LC-MS/MS is...